5x quick ligase buffer (New England Biolabs)
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New England Biolabs
5x quick ligase buffer
5x Quick Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+nebnext+quick+ligation+buffer/NEBNext+Quick+Ligation+RxBuff/pm42098827-396-20-24
Average 98 stars, based on 591 article reviews
5x Quick Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+nebnext+quick+ligation+buffer/NEBNext+Quick+Ligation+RxBuff/pm42098827-396-20-24
Average 98 stars, based on 591 article reviews
5x quick ligase buffer - by Bioz Stars,
2026-10
98/100 stars
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Ligation:Article Title: FTO depletion does not alter m 6 A stoichiometry in AML mRNA: a reassessment using direct RNA nanopore sequencing Article Snippet: MTT assay was performed following Abcam’s instructions, with 5 timepoints: 24 h, 48 h, 72 h, 96 h, and 168 h. On each day, one plate was sacrificed by spinning down at 1,000 x g for 5 min at 4°C, followed by aspiration and resuspension in 50 μl serum-free RPMI media and 50 μl of 5 mg/ml MTT solution (Abcam, ab146345-500mg) in PBS. .. Next, the pooled library was ligated with 6 μl RNA ligation adapter (RLA), 3 μl T4 DNA ligase, 8 μl Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μL Article Title: Parallel in-depth analysis of repeat expansions: an updated Clin-CATS workflow for nanopore R10 flow cells Article Snippet: .. To the now pooled samples, 10 μL Article Title: Chemical control of 2’-hydroxyl-dependent Cas9 target engagement enables CRISPR RNA ribose replacement Article Snippet: The pooled samples were purified using AMPure XP beads (Beckman Coulter) and eluted in 30 μL of elution buffer (10 mM Tris-HCl, pH 8.5). .. The eluted barcoded samples were adapter ligated by combining 30 μL of pooled barcoded samples, 5 μL of native adapter (NA), 10 μL of Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. In detail, for each sample, 100 ng of poly-adenylated in vitro transcribed RNA in 4.4 μL, was transferred into a PCR tube, followed by addition of 1 μL previously annealed custom RTA adapter, 1.6 μL Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: Reverse transcription was performed by adding 8 μL of 5X Induro buffer, 2 μL of 10 mM dNTP, 2 μL of 10 μM random hexamer oligonucleotides, 2 μL Induro RT (NEB), 0.5 μL RNasin in a final volume of 40 μL and incubation 2 min at 22°C (annealing), 15 min at 60°C (reverse transcription) and 10 min at 70°C (heat-inactivation). .. Briefly, 10 μL purified library was mixed with 6 μL Article Title: mRNA stability in response to m 6 A placement is linked to cell identity in planarians Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μl Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. For this we transferred 10 μL of purified reversetranscribed RNA-RTA library into a 1.5 ml DNA LoBind tube (Eppendorf), followed by addition of 2.5 μL H2O, 4 μL Concentration Assay:Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μL Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. In detail, for each sample, 100 ng of poly-adenylated in vitro transcribed RNA in 4.4 μL, was transferred into a PCR tube, followed by addition of 1 μL previously annealed custom RTA adapter, 1.6 μL Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: Reverse transcription was performed by adding 8 μL of 5X Induro buffer, 2 μL of 10 mM dNTP, 2 μL of 10 μM random hexamer oligonucleotides, 2 μL Induro RT (NEB), 0.5 μL RNasin in a final volume of 40 μL and incubation 2 min at 22°C (annealing), 15 min at 60°C (reverse transcription) and 10 min at 70°C (heat-inactivation). .. Briefly, 10 μL purified library was mixed with 6 μL Article Title: mRNA stability in response to m 6 A placement is linked to cell identity in planarians Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μl Incubation:Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μL Article Title: Parallel in-depth analysis of repeat expansions: an updated Clin-CATS workflow for nanopore R10 flow cells Article Snippet: .. To the now pooled samples, 10 μL Article Title: Chemical control of 2’-hydroxyl-dependent Cas9 target engagement enables CRISPR RNA ribose replacement Article Snippet: The pooled samples were purified using AMPure XP beads (Beckman Coulter) and eluted in 30 μL of elution buffer (10 mM Tris-HCl, pH 8.5). .. The eluted barcoded samples were adapter ligated by combining 30 μL of pooled barcoded samples, 5 μL of native adapter (NA), 10 μL of Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: Reverse transcription was performed by adding 8 μL of 5X Induro buffer, 2 μL of 10 mM dNTP, 2 μL of 10 μM random hexamer oligonucleotides, 2 μL Induro RT (NEB), 0.5 μL RNasin in a final volume of 40 μL and incubation 2 min at 22°C (annealing), 15 min at 60°C (reverse transcription) and 10 min at 70°C (heat-inactivation). .. Briefly, 10 μL purified library was mixed with 6 μL Article Title: mRNA stability in response to m 6 A placement is linked to cell identity in planarians Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μl Purification:Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μL Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: Reverse transcription was performed by adding 8 μL of 5X Induro buffer, 2 μL of 10 mM dNTP, 2 μL of 10 μM random hexamer oligonucleotides, 2 μL Induro RT (NEB), 0.5 μL RNasin in a final volume of 40 μL and incubation 2 min at 22°C (annealing), 15 min at 60°C (reverse transcription) and 10 min at 70°C (heat-inactivation). .. Briefly, 10 μL purified library was mixed with 6 μL Article Title: mRNA stability in response to m 6 A placement is linked to cell identity in planarians Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μl Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. For this we transferred 10 μL of purified reversetranscribed RNA-RTA library into a 1.5 ml DNA LoBind tube (Eppendorf), followed by addition of 2.5 μL H2O, 4 μL Next-Generation Sequencing:Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μL Article Title: mRNA stability in response to m 6 A placement is linked to cell identity in planarians Article Snippet: .. For each sample, 100 ng of polyA-selected RNA were ligated to 1 μL of 0.7 μM annealed RTA in the presence of 2 μl In Vitro:Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. In detail, for each sample, 100 ng of poly-adenylated in vitro transcribed RNA in 4.4 μL, was transferred into a PCR tube, followed by addition of 1 μL previously annealed custom RTA adapter, 1.6 μL Polymerase Chain Reaction:Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing Article Snippet: .. In detail, for each sample, 100 ng of poly-adenylated in vitro transcribed RNA in 4.4 μL, was transferred into a PCR tube, followed by addition of 1 μL previously annealed custom RTA adapter, 1.6 μL Sequencing:Article Title: Complete post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species Article Snippet: Reverse transcription was performed by adding 8 μL of 5X Induro buffer, 2 μL of 10 mM dNTP, 2 μL of 10 μM random hexamer oligonucleotides, 2 μL Induro RT (NEB), 0.5 μL RNasin in a final volume of 40 μL and incubation 2 min at 22°C (annealing), 15 min at 60°C (reverse transcription) and 10 min at 70°C (heat-inactivation). .. Briefly, 10 μL purified library was mixed with 6 μL |